Did Leonardo da Vinci paint two Mona Lisas? This documentary explores the ‘two-painting theory’ and presents decades of research and scientific testing surrounding the ‘Isleworth Mona Lisa’. Historical facts dating back to the early sixteenth century point to the existence of a second, earlier Mona Lisa portrait by da Vinci. In 1913, renowned art dealer Hugh Blaker discovered the painting in an English manor house. He and several others championed the painting’s authentication for many years to come, until it was ultimately locked away in a Swiss bank vault for nearly forty years. Today, the non-profit Mona Lisa Foundation is tasked with proving this stunning painting’s authenticity. After amassing more than three decades of scientific studies - from carbon testing to multispectral digitization - and piecing together five hundred years of clues, the Foundation prepares to unveil this masterpiece to the world.